8H18
Crystal structure of DnaQ domain of Streptococcus thermophilus strain DGCC 7710
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97855 |
Spacegroup name | H 3 |
Unit cell lengths | 96.298, 96.298, 61.455 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.505 - 1.499 |
R-factor | 0.1764 |
Rwork | 0.176 |
R-free | 0.20070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j53 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.499 | 4.070 | 1.500 |
Rmerge | 0.193 | 0.155 | 1.002 |
Rmeas | 0.203 | 0.163 | 1.078 |
Rpim | 0.063 | 0.049 | 0.387 |
Number of reflections | 34181 | 1708 | 1706 |
<I/σ(I)> | 6.5 | ||
Completeness [%] | 100.0 | 100 | 99.9 |
Redundancy | 9.8 | 10.7 | 6.8 |
CC(1/2) | 0.987 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM ammonium sulfate, 36% (w/v) PEG8000, and 100 mM sodium cacodylate trihydrate pH 6.5 |