8H0T

Crystal structure of MnmM from B. subtilis complexed with SAH (1.17 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97926
Spacegroup name	C 1 2 1
Unit cell lengths	52.555, 55.387, 60.592
Unit cell angles	90.00, 103.16, 90.00

Refinement procedure

Resolution	37.590 - 1.170
R-factor	0.13642
Rwork	0.135
R-free	0.16663
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pon
RMSD bond length	0.012
RMSD bond angle	1.712
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	STARANISO
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.590	1.300
High resolution limit [Å]	1.160	1.160
Rmerge	0.098	0.807
Rmeas	0.106	0.951
Rpim	0.041	0.493
Number of reflections	41988	2103
<I/σ(I)>	11.6	1.6
Completeness [%]	72.5
Redundancy	6.5
CC(1/2)	0.999	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES pH 7.5, 25% w/v PEG 3,350