8H0N

Crystal structure of the human METTL1-WDR4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 2 21 21
Unit cell lengths	84.465, 87.030, 95.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.680 - 1.800
R-factor	0.1948
Rwork	0.193
R-free	0.22650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5il2
RMSD bond length	0.006
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.029	0.111
Rmeas	0.041	0.156
Rpim	0.029	0.111
Number of reflections	66185	13017
<I/σ(I)>	15.03	5.57
Completeness [%]	99.9	99.98
Redundancy	2	2
CC(1/2)	0.997	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Bis-Tris, Lithium sulphate, PEG3350.