8H0M
Crystal structure of VioD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2013-05-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 64 |
Unit cell lengths | 90.039, 90.039, 94.582 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.670 - 1.702 |
Rwork | 0.205 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4a |
RMSD bond length | 0.009 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (v1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.670 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rpim | 0.035 | 0.464 |
Number of reflections | 47638 | 2501 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 99.9 | |
Redundancy | 11 | |
CC(1/2) | 0.996 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | Na Formate |