8H0F

Crystal structure of collagen heterotrimer with KD,ER and KE axial pairs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	C 1 2 1
Unit cell lengths	47.362, 25.829, 68.424
Unit cell angles	90.00, 104.41, 90.00

Refinement procedure

Resolution	23.560 - 1.870
R-factor	0.1758
Rwork	0.174
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yan
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.560	1.900
High resolution limit [Å]	1.870	1.870
Rmerge	0.039	0.039
Number of reflections	6459	6455
<I/σ(I)>	63.18
Completeness [%]	94.1
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% PEG3350 MME, 0.1M Trisodium citrate dihydrate, pH5.0