8H0E
Crystal structure of collagen heterotrimer with KD, ER and KE axial pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 61.067, 42.411, 28.749 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 29.260 - 1.760 |
| R-factor | 0.2261 |
| Rwork | 0.225 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yan |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.260 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.770 |
| Rmerge | 0.103 | 0.103 |
| Number of reflections | 6945 | 6884 |
| <I/σ(I)> | 22.47 | |
| Completeness [%] | 96.6 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% isopropanol, 0.1M Bicine pH 8.5, 30% PEG3350 |
