8H09
Structure of the thermolabile hemolysin from Vibrio alginolyticus (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97876 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.932, 71.238, 83.645 |
Unit cell angles | 90.00, 101.32, 90.00 |
Refinement procedure
Resolution | 53.780 - 1.810 |
R-factor | 0.181 |
Rwork | 0.180 |
R-free | 0.20400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6jkz |
RMSD bond length | 0.010 |
RMSD bond angle | 0.930 |
Data reduction software | XDS (Mar 15, 2019, built on 20190315) |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.783 | 1.840 |
High resolution limit [Å] | 1.809 | 1.809 |
Rmeas | 0.087 | 0.767 |
Rpim | 0.033 | 0.288 |
Number of reflections | 68600 | 3412 |
<I/σ(I)> | 12.9 | 2.3 |
Completeness [%] | 98.8 | 98.1 |
Redundancy | 6.9 | 6.9 |
CC(1/2) | 0.998 | 0.863 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | The crystal of ValTLH was grown in a drop containing 1.0 ul of protein solution (10 mg/ml in the buffer 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 200 mM NDSB201) and 1.0 ul of reservoir solution (0.1 M Tris pH 8.5, 20.5% polyethylene glycol 4000, 20% polyethylene glycol 400, and 0.1 M MgCl2). |