8H02

Crystal structure of Synechococcus elongatus PCC 7942 RNA polymerase SI3-tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	32.133, 32.133, 82.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.133 - 1.552
R-factor	0.2
Rwork	0.198
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2auk
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.066	0.165
Number of reflections	11917	609
<I/σ(I)>	26.3
Completeness [%]	98.5
Redundancy	7.2
CC(1/2)	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	0.2 M Ammonium acetate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350