8H02
Crystal structure of Synechococcus elongatus PCC 7942 RNA polymerase SI3-tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-24 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 |
Unit cell lengths | 32.133, 32.133, 82.399 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.133 - 1.552 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2auk |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.066 | 0.165 |
Number of reflections | 11917 | 609 |
<I/σ(I)> | 26.3 | |
Completeness [%] | 98.5 | |
Redundancy | 7.2 | |
CC(1/2) | 0.981 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M Ammonium acetate, 0.1 M Tris pH 8.5, 25% w/v Polyethylene glycol 3,350 |