8GZX

Escherichia coli FtsZ complexed with monobody (P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	45.105, 63.301, 123.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.200 - 1.840
R-factor	0.2071
Rwork	0.205
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ll5
RMSD bond length	0.007
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.200	1.906
High resolution limit [Å]	1.840	1.840
Number of reflections	31362	3066
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	13.1
CC(1/2)	0.999	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M sodium formate, 0.1M Bis-Tris propane pH6.5, 20% PEG 3350