8GZV

Klebsiella pneumoniae FtsZ complexed with monobody (P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	45.285, 64.563, 124.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.540 - 2.200
R-factor	0.2076
Rwork	0.204
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ll5
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.540	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	19223	1877
<I/σ(I)>	17.67
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M sodium formate, 0.1M Bis-Tris propane pH6.5, 20% PEG 3350