8GYI

Crystal structure of Fic25 (holo form) from Streptomyces ficellus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	201.628, 201.628, 57.009
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.480 - 1.930
R-factor	0.1677
Rwork	0.167
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gyh
RMSD bond length	0.018
RMSD bond angle	1.244
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.120	0.900
Number of reflections	99447	4853
<I/σ(I)>	17.3	2.8
Completeness [%]	99.5
Redundancy	10.2
CC(1/2)	0.998	0.817

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	1.0 M Sodium citrate, 0.1 M Imidazole, pH 8.0