8GYI
Crystal structure of Fic25 (holo form) from Streptomyces ficellus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2021-12-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 201.628, 201.628, 57.009 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.480 - 1.930 |
R-factor | 0.1677 |
Rwork | 0.167 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8gyh |
RMSD bond length | 0.018 |
RMSD bond angle | 1.244 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.480 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.120 | 0.900 |
Number of reflections | 99447 | 4853 |
<I/σ(I)> | 17.3 | 2.8 |
Completeness [%] | 99.5 | |
Redundancy | 10.2 | |
CC(1/2) | 0.998 | 0.817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 1.0 M Sodium citrate, 0.1 M Imidazole, pH 8.0 |