8GYH
Crystal structure of Fic25 (apo form) from Streptomyces ficellus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 201.658, 201.658, 56.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.480 - 1.800 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model constructed by AlphaFold2 from the primary amino acid sequence of Fic25 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.932 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.484 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.112 | 0.940 |
| Number of reflections | 122992 | 6059 |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.999 | 0.835 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1.0 M Sodium citrate, 0.1 M Imidazole, pH8.0 |
