8GYG
Purification ,Crystallization and X-ray Diffraction analysis of a novel arysulfatase from Pseudoalteromonas atlantica T6c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.170, 61.380, 89.600 |
Unit cell angles | 90.00, 97.88, 90.00 |
Refinement procedure
Resolution | 29.585 - 2.000 |
R-factor | 0.2025 |
Rwork | 0.200 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nks |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.610 | 1.590 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.117 | 1.077 |
Number of reflections | 85833 | 6332 |
<I/σ(I)> | 8.3 | |
Completeness [%] | 99.7 | |
Redundancy | 6.1 | |
CC(1/2) | 0.995 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 2o%PEG 3350, 0.2M Sodium Thiocyamate |