8GYB

Crystal structure of Alongshan virus methyltransferase bound to S-adenosyl-L-homocysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	90.841, 170.508, 172.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.182 - 2.101
R-factor	0.2112
Rwork	0.210
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gy4
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.134	0.409
Number of reflections	77683	7649
<I/σ(I)>	13.297
Completeness [%]	100.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris pH 8.5, 3.0M Sodium chloride