8GY9

Crystal structure of Alongshan virus methyltransferase bound to S-adenosyl-L-methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 42 21 2
Unit cell lengths	119.572, 119.572, 87.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.163 - 2.300
R-factor	0.1938
Rwork	0.192
R-free	0.24110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gy4
RMSD bond length	0.008
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.129	0.966
Number of reflections	28864	2835
<I/σ(I)>	11.529
Completeness [%]	99.7
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.5M Lithium sulfate, 0.1M Tris pH 8.5, 25% w/v PEG 3350