8GY1
Crystal structure of Ag+ binding to Dendrorhynchus zhejiangensis ferritin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL17B |
Synchrotron site | NFPSS |
Beamline | BL17B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-25 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.979 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 148.212, 148.212, 148.212 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 104.800 - 1.900 |
R-factor | 0.15273 |
Rwork | 0.151 |
R-free | 0.18965 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.875 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | BALBES |
Refinement software | REFMAC (ccp4-7.0.024) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 106.780 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 21003 | 3175 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.7 | |
Redundancy | 11.3 | |
CC(1/2) | 0.990 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291.15 | DzFer crystals were grown in 0.2 M ammonium sulfate, 0.1 M sodium acetate trihydrate pH 4.6, 30% w/v PEG monomethyl ether 2000, followed by soaking for 10 min with 20% glycerol and 100 mM AgNO3. |