8GXV
Structure of a bacterial serpin Choloropin derived from Cholorobium limicola
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 60.660, 60.910, 130.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.910 - 2.200 |
R-factor | 0.2033 |
Rwork | 0.201 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ee5 |
Data scaling software | Aimless |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.910 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.030 | |
Number of reflections | 25206 | 2453 |
<I/σ(I)> | 15.11 | |
Completeness [%] | 99.9 | |
Redundancy | 2 | |
CC(1/2) | 0.999 | 0.959 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 25% PEG MME 550, 0.1M MES pH 6.5, 0.01M Zinc Sulfate |