8GXR

crystal structure of UBC domain of UBE2O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979191
Spacegroup name	P 21 21 21
Unit cell lengths	64.390, 96.960, 207.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.090 - 1.700
R-factor	0.2258
Rwork	0.225
R-free	0.24990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	none
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.090	1.740
High resolution limit [Å]	1.700	1.700
Number of reflections	143060	10459
<I/σ(I)>	11.6
Completeness [%]	99.9
Redundancy	12.9
CC(1/2)	0.998	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	sodium citrate