8GXO
The crystal structure of CsFAOMT1 in complex with SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CAMD BEAMLINE GCPCC |
Synchrotron site | CAMD |
Beamline | GCPCC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-26 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.978690 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.899, 99.899, 99.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.260 - 1.740 |
R-factor | 0.1869 |
Rwork | 0.186 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c3y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.016 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Number of reflections | 53776 | 847 |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.0 | |
Redundancy | 20 | |
CC(1/2) | 1.000 | 0.855 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M Cadmium Sulfate hydrate, 0.1 M HEPES pH 7.5, 1.0 M Sodium Acetate |