8GXL
HUMAN SUGP1 433-577
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 61 |
Unit cell lengths | 59.657, 59.657, 178.404 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.660 - 2.400 |
R-factor | 0.2044 |
Rwork | 0.198 |
R-free | 0.26100 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.935 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.660 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 14002 | 1405 |
<I/σ(I)> | 16.9 | |
Completeness [%] | 99.9 | |
Redundancy | 5.2 | |
CC(1/2) | 0.997 | 0.835 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium tartrate dibasic, pH 7.0, and 20% (w/v) polyethylene glycol 3350. |