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8GXL

HUMAN SUGP1 433-577

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2019-06-29
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9792
Spacegroup nameP 61
Unit cell lengths59.657, 59.657, 178.404
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution51.660 - 2.400
R-factor0.2044
Rwork0.198
R-free0.26100
Structure solution methodSAD
RMSD bond length0.008
RMSD bond angle0.935
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHENIX (1.17.1_3660)
Refinement softwarePHENIX (1.17.1_3660)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]51.6602.490
High resolution limit [Å]2.4002.400
Number of reflections140021405
<I/σ(I)>16.9
Completeness [%]99.9
Redundancy5.2
CC(1/2)0.9970.835
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP293.150.2 M ammonium tartrate dibasic, pH 7.0, and 20% (w/v) polyethylene glycol 3350.

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