8GXF
Pseudomonas flexibilis GCN5 family acetyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.889, 99.013, 98.822 |
Unit cell angles | 90.00, 101.87, 90.00 |
Refinement procedure
Resolution | 42.950 - 3.040 |
R-factor | 0.2328 |
Rwork | 0.225 |
R-free | 0.30190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yre |
RMSD bond length | 0.022 |
RMSD bond angle | 2.104 |
Data reduction software | HKL-2000 (v1.0) |
Data scaling software | HKL-2000 (v1.0) |
Phasing software | PHENIX (1.20.1) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.950 | 3.150 |
High resolution limit [Å] | 3.040 | 3.040 |
Rmerge | 0.471 | 0.511 |
Number of reflections | 30921 | 15925 |
<I/σ(I)> | 34.1 | |
Completeness [%] | 99.6 | |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M NaCl, 0.1M MES pH6.5, 10% PEG4000 |