8GXD
L-LEUCINE DEHYDROGENASE FROM EXIGUOBACTERIUM SIBIRICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-02 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.54178 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 152.990, 152.990, 132.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.620 - 3.020 |
R-factor | 0.2156 |
Rwork | 0.213 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6acf |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.620 | 3.200 |
High resolution limit [Å] | 3.020 | 3.020 |
Number of reflections | 15638 | 2418 |
<I/σ(I)> | 9.5 | |
Completeness [%] | 99.5 | |
Redundancy | 5.2 | |
CC(1/2) | 0.986 | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M calcium chloride, 0.1M sodium acetate buffer (pH 4.6), 20% glycerol (w/v) |