8GX9

Crystal structure of SARS-CoV-2 RBD with P2C-1F11 and P2B-1G5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9769
Spacegroup name	P 21 21 21
Unit cell lengths	110.511, 154.856, 158.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.080 - 4.010
R-factor	0.2411
Rwork	0.238
R-free	0.29690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cdi
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.080	4.153
High resolution limit [Å]	4.010	4.010
Rmerge	0.290
Number of reflections	23031	2097
<I/σ(I)>	4.8
Completeness [%]	97.3
Redundancy	6.3
CC(1/2)		0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M sodium chloride, 0.1M MES, pH 6.0, 20% PEG 2000 MME