8GVI
The complex between H25-11 TCR and HLA-A24 bound to HIV-1 Nef138-8 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 70.524, 70.524, 384.253 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.810 - 3.300 |
R-factor | 0.2879 |
Rwork | 0.286 |
R-free | 0.31350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxm |
RMSD bond length | 0.002 |
RMSD bond angle | 0.545 |
Data reduction software | xia2 |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.161 | 2.033 |
Number of reflections | 17740 | 17740 |
<I/σ(I)> | 18.477 | 1.406 |
Completeness [%] | 99.5 | |
Redundancy | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Potassium chloride, 0.1 M tris (pH 8.0), 15% PEG2000 MME |