8GVG
The complex between public TCR TD08 and HLA-A24 bound to HIV-1 Nef138-8 (2F) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.035, 71.035, 383.869 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.940 - 3.370 |
| R-factor | 0.3004 |
| Rwork | 0.299 |
| R-free | 0.31990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vxm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | xia2 |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.480 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rmerge | 0.127 | 0.968 |
| Number of reflections | 16829 | 16829 |
| <I/σ(I)> | 22.933 | 5.539 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 16.9 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Sodium sulfate, 0.1 M Bis-Tris Propane (pH 7.5), 20% PEG 3350 |
