8GV6

Crystal structure of PN-SIA28 in complex with influenza hemagglutinin H14 (A/long-tailed duck/Wisconsin/10OS3912/2010)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97890
Spacegroup name	P 1 21 1
Unit cell lengths	91.662, 120.990, 129.759
Unit cell angles	90.00, 107.73, 90.00

Refinement procedure

Resolution	49.720 - 3.400
R-factor	0.2232
Rwork	0.221
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjs
RMSD bond length	0.003
RMSD bond angle	0.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.520
High resolution limit [Å]	3.400	3.400
Rmerge	0.300	1.300
Number of reflections	36855	3591
<I/σ(I)>	5.58	1.1
Completeness [%]	87.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Tris, 5% w/v PGA-LM, 20% w/v PEG3350