8GV4

A human broadly neutralizing influenza A hemagglutinin stem-specific antibody PN-SIA28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-06
Detector	ADSC QUANTUM 315
Wavelength(s)	1.03906
Spacegroup name	P 1 21 1
Unit cell lengths	76.560, 86.361, 82.895
Unit cell angles	90.00, 105.29, 90.00

Refinement procedure

Resolution	42.140 - 2.500
R-factor	0.1967
Rwork	0.195
R-free	0.22980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjs
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.120	0.390
Number of reflections	36196	7086
<I/σ(I)>	15.4	7.9
Completeness [%]	97.9
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES pH7.0, 12% PEG 3350