8GUP
Crystal structure of EsaG from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978690 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.674, 62.106, 82.811 |
| Unit cell angles | 90.00, 117.49, 90.00 |
Refinement procedure
| Resolution | 49.158 - 2.299 |
| R-factor | 0.219855608258 |
| Rwork | 0.216 |
| R-free | 0.25342 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.576 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.300 |
| Number of reflections | 35477 | 35477 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 7.7 | |
| CC(1/2) | 1.000 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M sodium phosphate/citric acid (pH4.2), 10% 3350 0.3M NaCl |
