8GUM
Chitin-active AA10 LPMO (GbpA) from Vibrio campbellii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-04-23 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.99764 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 113.612, 113.612, 215.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.100 - 2.350 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xwx |
RMSD bond length | 0.009 |
RMSD bond angle | 0.995 |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.092 | 1.595 |
Rmeas | 0.096 | 1.680 |
Rpim | 0.025 | 0.514 |
Number of reflections | 59698 | 2937 |
<I/σ(I)> | 28 | 0.8 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 14.3 | 10 |
CC(1/2) | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 1.0M Ammonium phosphate dibasic; 0.1M ADA buffer, pH 6.5 |