8GU1
Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with Pimozide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-17 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 90.014, 45.795, 115.675 |
Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.650 |
R-factor | 0.2392 |
Rwork | 0.237 |
R-free | 0.28280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a80 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.932 |
Data reduction software | CrysalisPro |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 9.010 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 29525 | 3590 |
<I/σ(I)> | 5.5 | |
Completeness [%] | 99.8 | |
Redundancy | 4.3 | |
CC(1/2) | 0.970 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Sodium citrate, 20% PEG 550, pH 5 |