8GTX
Crystal Structure of human Spindlin1-HBx complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.788, 67.096, 93.695 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.850 - 1.800 |
R-factor | 0.1647 |
Rwork | 0.163 |
R-free | 0.19670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mzg |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.101 | 0.048 | 0.577 |
Rmeas | 0.110 | 0.052 | 0.627 |
Rpim | 0.043 | 0.021 | 0.243 |
Total number of observations | 185436 | ||
Number of reflections | 28755 | 1589 | 1431 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 99.9 | 99.7 | 100 |
Redundancy | 6.4 | 6 | 6.5 |
CC(1/2) | 0.997 | 0.905 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 16%(w/v) PEG 8000, 0.04M Potassium phosphate dibasic, 20% (v/v) Glycerol |