8GSX
X-ray structure of Clostridium perfringens pili protein B collagen-binding domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 127.390, 127.390, 163.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.830 - 2.860 |
| R-factor | 0.25402 |
| Rwork | 0.252 |
| R-free | 0.28590 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.673 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.830 | 2.930 |
| High resolution limit [Å] | 2.860 | 2.860 |
| Number of reflections | 18588 | 1330 |
| <I/σ(I)> | 10.13 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.994 | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100 mM sodium acetate pH4.8, 400 mM ammonium sulfate, 16-19% w/v PEG400 |
