8GRJ
Crystal structure of gamma-alpha subunit complex from Burkholderia cepacia FAD glucose dehydrogenase in complex with gluconolactone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 111.235, 111.235, 527.106 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.040 - 2.950 |
R-factor | 0.2018 |
Rwork | 0.198 |
R-free | 0.27020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6a2u |
RMSD bond length | 0.007 |
RMSD bond angle | 1.705 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.010 | 2.950 |
Rmerge | 0.113 | 0.054 | 0.424 |
Total number of observations | 653811 | ||
Number of reflections | 42134 | 2284 | 2055 |
<I/σ(I)> | 7.4 | ||
Completeness [%] | 99.4 | 93 | 100 |
Redundancy | 15.5 | 13.6 | 16.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Tacsimate |