8GQW
The Crystal Structures of a Swine SLA-2*HB01 Molecules Complexed with a CTL epitope from Asia1 serotype of Foot-and-mouth disease virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-05-04 |
Detector | MAATEL IMAGINE |
Wavelength(s) | 0.97892 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.626, 98.458, 166.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.558 - 2.480 |
R-factor | 0.2157 |
Rwork | 0.213 |
R-free | 0.26020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q94 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.149 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.480 | 2.500 |
Rmerge | 0.124 | 0.124 |
Number of reflections | 28939 | |
<I/σ(I)> | 13.879 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | Ammonium acetate, PEG, BIS-TRIS |