8GQW

The Crystal Structures of a Swine SLA-2*HB01 Molecules Complexed with a CTL epitope from Asia1 serotype of Foot-and-mouth disease virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-04
Detector	MAATEL IMAGINE
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	48.626, 98.458, 166.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.558 - 2.480
R-factor	0.2157
Rwork	0.213
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q94
RMSD bond length	0.006
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.480	2.500
Rmerge	0.124	0.124
Number of reflections	28939
<I/σ(I)>	13.879
Completeness [%]	99.7	99.7
Redundancy	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	Ammonium acetate, PEG, BIS-TRIS