8GQR
Crystal structure of VioD with FAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.603, 100.869, 162.815 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.770 - 2.100 |
Rwork | 0.190 |
R-free | 0.22560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4a |
RMSD bond length | 0.008 |
RMSD bond angle | 0.943 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (v1.16) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.770 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.130 | 0.677 |
Number of reflections | 49417 | 3951 |
<I/σ(I)> | 6.2 | |
Completeness [%] | 99.7 | |
Redundancy | 7.3 | |
CC(1/2) | 0.982 | 0.499 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG 3350 |