8GQQ
The three-dimensional structure of Cystatin A2, a cysteine protease inhibitor from Dictyostelium discoideum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-02-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 59.273, 63.274, 24.911 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.911 - 1.069 |
R-factor | 0.1793 |
Rwork | 0.179 |
R-free | 0.18990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kfq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.911 | 1.107 |
High resolution limit [Å] | 1.069 | 1.069 |
Rmerge | 0.128 | 0.390 |
Number of reflections | 42018 | 4113 |
<I/σ(I)> | 10.8 | |
Completeness [%] | 99.0 | |
Redundancy | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 22% (w/v) PEG 2K, 100 mM MES pH 6.5, 60 mM sodium acetate, 10 mM TCEP |