8GPT

YFV_E_YD6scFv_postfusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	228.536, 150.866, 126.418
Unit cell angles	90.00, 112.00, 90.00

Refinement procedure

Resolution	49.620 - 3.070
R-factor	0.2129
Rwork	0.211
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jhl
RMSD bond length	0.004
RMSD bond angle	0.672
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.070	3.100
Number of reflections	70474	6984
<I/σ(I)>	10.3
Completeness [%]	98.7
Redundancy	5.5
CC(1/2)	0.995	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	in 0.1M ammonium sulfate, 0.3M sodium formate, 0.1M sodium acetate, 3% (w/v) PGA-LM, 5% (w/v) PEG 8,000, pH5.0