8GPS

Cytoplasmic domain structure of the MgtE Mg2+ channel from Chryseobacterium hispalense

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	293
Detector technology	PIXEL
Collection date	2020-06-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	57.503, 57.503, 118.252
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.860 - 1.390
R-factor	0.1862
Rwork	0.184
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yvy
RMSD bond length	0.006
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	1.460
High resolution limit [Å]	1.390	1.390
Number of reflections	46278	6708
<I/σ(I)>	20.4
Completeness [%]	99.9
Redundancy	18
CC(1/2)	0.990	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.1 M sodium chloride, 0.1 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 20% v/v pentaerythritol