8GPS
Cytoplasmic domain structure of the MgtE Mg2+ channel from Chryseobacterium hispalense
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2020-06-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.503, 57.503, 118.252 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.860 - 1.390 |
R-factor | 0.1862 |
Rwork | 0.184 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yvy |
RMSD bond length | 0.006 |
RMSD bond angle | 0.860 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.800 | 1.460 |
High resolution limit [Å] | 1.390 | 1.390 |
Number of reflections | 46278 | 6708 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 99.9 | |
Redundancy | 18 | |
CC(1/2) | 0.990 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1 M sodium chloride, 0.1 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 20% v/v pentaerythritol |