8GOT
Crystal structure of wild-type protease 3C from Seneca Valley Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9788 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.303, 69.496, 75.504 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.086 - 1.989 |
R-factor | 0.2151 |
Rwork | 0.213 |
R-free | 0.25320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6l0t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.919 |
Data reduction software | autoPROC |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 1.989 | 1.990 |
Rmerge | 0.130 | 0.752 |
Rmeas | 0.130 | 0.766 |
Rpim | 0.060 | |
Number of reflections | 25880 | 4279 |
<I/σ(I)> | 17 | 1.8 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 35.9 | 20.8 |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG 3350, 1% (w/v) tryptone, 0.05% (w/v) sodium azide |