8GOP
SARS-CoV-2 specific private TCR RLQ7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.178, 68.054, 63.878 |
Unit cell angles | 90.00, 90.24, 90.00 |
Refinement procedure
Resolution | 34.800 - 2.800 |
R-factor | 0.2333 |
Rwork | 0.231 |
R-free | 0.28620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8gom |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.800 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 10990 | 1107 |
<I/σ(I)> | 9.39 | |
Completeness [%] | 94.5 | |
Redundancy | 9.39 | |
CC(1/2) | 0.842 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.02 M Sodium phosphate monobasic, 0.1M Bis-Tris propane pH 7.5, 20% PEG 3350 |