8GON

SARS-CoV-2 specific private TCR RLQ7 in complex with RLQ-T1006I-HLA-A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	147.366, 147.366, 178.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.237 - 2.601
R-factor	0.2009
Rwork	0.198
R-free	0.25270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8gom
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.240	2.694
High resolution limit [Å]	2.600	2.600
Rmerge	0.113
Number of reflections	35792	3505
<I/σ(I)>	22
Completeness [%]	99.9
Redundancy	25.1
CC(1/2)	0.994	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl pH 8.5, 15% PEG 6000