8GOK
Legionella OTU deubiquitinase LotA OTU1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97942 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 86.217, 86.217, 91.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.480 - 1.520 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.21330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7f9x |
RMSD bond length | 0.006 |
RMSD bond angle | 0.905 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.480 | 1.574 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.017 | 0.457 |
Rmeas | 0.025 | 0.646 |
Rpim | 0.017 | 0.457 |
Number of reflections | 53716 | 5296 |
<I/σ(I)> | 18.91 | 1.56 |
Completeness [%] | 100.0 | 99.94 |
Redundancy | 2 | 2 |
CC(1/2) | 1.000 | 0.641 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 290 | 35% (w/v) PEG 4000, 0.1M Tris-HCl [pH7.8], 0.8 M Lithium Chloride |