8GO5

Fungal immunomodulatory protein FIP-nha WT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-25
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 1
Unit cell lengths	66.577, 74.467, 87.473
Unit cell angles	99.33, 112.15, 108.59

Refinement procedure

Resolution	25.686 - 2.809
R-factor	0.2206
Rwork	0.219
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wdl
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0123)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.809	2.809
Rmerge	0.082	0.320
Number of reflections	33042	1675
<I/σ(I)>	13.91	2.41
Completeness [%]	96.8
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	22% PEG 1000, 0.1M Sodium citrate pH3.5, 0.2M Ammonium sulfate