8GN9

SPFH domain of Pyrococcus horikoshii stomatin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2020-10-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98000
Spacegroup name	P 41 21 2
Unit cell lengths	28.014, 28.014, 240.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.500
R-factor	0.2217
Rwork	0.215
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bk6
RMSD bond length	0.007
RMSD bond angle	1.381
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.140	0.311
Number of reflections	3909	536
<I/σ(I)>	10.6	6.8
Completeness [%]	99.7	100
Redundancy	10.3
CC(1/2)	0.995	0.951

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M sodium acetate, 50 mM Tris-HCl, 15% PEG4000