8GN2
Crystal structure of PPBQ-bound photosystem II complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-04-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 124.018, 229.579, 287.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.220 - 1.950 |
R-factor | 0.154 |
Rwork | 0.153 |
R-free | 0.18140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wu2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.214 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.061 | 1.465 |
Number of reflections | 583631 | 57320 |
<I/σ(I)> | 18.6 | 1.4 |
Completeness [%] | 98.7 | 97.6 |
Redundancy | 7 | 7 |
CC(1/2) | 1.000 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.1 | 285 | 5% PEG1450, 20mM NaCl, 10mM CaCl2, 40mM MgSO4, 20mM MES buffer, pH 6.1, MICRO-BATCH METHOD UNDER OIL, temperature 285K |