8GMZ
Structure of methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.969, 97.969, 41.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.420 - 1.880 |
| R-factor | 0.2126 |
| Rwork | 0.211 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.375 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.840 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 18348 | 2709 |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 97.8 | |
| Redundancy | 15.7 | |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 291.15 | Sodium citrate tribasic dihydrate pH 5.0, Jeffamine ED-2001 |
