8GMV
Crystal structure of lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-07-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97933 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.441, 79.441, 37.113 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.970 - 2.200 |
R-factor | 0.1894 |
Rwork | 0.184 |
R-free | 0.23410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wuc |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.970 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 6329 | 346 |
<I/σ(I)> | 15.54 | |
Completeness [%] | 98.9 | |
Redundancy | 17.9 | |
CC(1/2) | 0.973 | 0.666 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.6 | 298 | Na-acetate, NaCl, PEG 8000 |