8GLR
FrlB - Deglycase for 6-phosphofructose lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-19 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.680, 75.223, 82.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.090 - 1.830 |
| R-factor | 0.16209 |
| Rwork | 0.160 |
| R-free | 0.20331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a3n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.655 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.083 | 0.462 |
| Rmeas | 0.091 | 0.511 |
| Rpim | 0.038 | 0.214 |
| Number of reflections | 28584 | 28564 |
| <I/σ(I)> | 20.5 | 3.7 |
| Completeness [%] | 94.3 | 84.7 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M HEPES (pH 7.5) and 1.0 M ammonium sulfate |
