8GKP
Crystal Structure Analysis of Aspergillus fumigatus alkaline protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.092, 75.218, 87.929 |
| Unit cell angles | 90.00, 101.39, 90.00 |
Refinement procedure
| Resolution | 30.200 - 1.550 |
| R-factor | 0.1556 |
| Rwork | 0.153 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.515 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 86.197 | 37.609 | 1.630 |
| High resolution limit [Å] | 1.550 | 4.900 | 1.550 |
| Rmerge | 0.026 | 0.945 | |
| Rmeas | 0.074 | 0.030 | 1.099 |
| Rpim | 0.037 | 0.015 | 0.553 |
| Total number of observations | 355823 | ||
| Number of reflections | 91847 | 3007 | 13092 |
| <I/σ(I)> | 10.9 | 20 | 0.8 |
| Completeness [%] | 97.3 | 97.3 | 95.6 |
| Redundancy | 3.9 | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | polyethyleneglycol 550 monomethylether, polyethyleneglycol 20,000, MES/Imidazole |
