8GKO
Crystal Structure Analysis of Aspergillus fumigatus alkaline protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.605, 70.765, 78.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.580 - 1.060 |
| R-factor | 0.1412 |
| Rwork | 0.140 |
| R-free | 0.16000 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.384 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | Arcimboldo |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.577 | 35.382 | 1.120 |
| High resolution limit [Å] | 1.060 | 3.350 | 1.060 |
| Rmerge | 0.033 | 1.125 | |
| Rmeas | 0.088 | 0.038 | 1.450 |
| Rpim | 0.043 | 0.018 | 0.903 |
| Total number of observations | 549701 | 22128 | 28789 |
| Number of reflections | 142896 | 5144 | 14274 |
| <I/σ(I)> | 8.6 | 33.6 | 0.7 |
| Completeness [%] | 93.5 | 99.1 | 65.2 |
| Redundancy | 3.8 | 4.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | polyethyleneglycol 550 monomethylether, polyethyleneglycol 20,000, MES/Imidazole buffer |
